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How The Testing Was Done

        Five test solutions were chosen that were known to have total nitrite or nitrate levels in the range of aquarium nitrite and nitrate test kits. These were: 1) A sample of "typical" aquarium water of NO21- or NO31- < 0.05 ppm (as a blank), 2) a sodium nitrite (NaNO2) solution with NO21- = 1.0 ppm, or a sodium nitrate (NaNO3) solution with NO31- = 20.0 ppm, 3) a sodium nitrite (NaNO2) solution with NO21- = 2.0 ppm, or a sodium nitrate (NaNO3) solution with NO31- = 75.0 ppm, 4) a sodium nitrite (NaNO2) solution with NO21- = 3.0 ppm, or a sodium nitrate (NaNO3) solution with NO31- = 100.0 ppm, and 5) a sodium nitrite (NaNO2) solution with NO21- = 4.0 ppm, or a sodium nitrate (NaNO3) solution with NO31- = 125.0 ppm. The sodium nitrite and sodium nitrate solutions were prepared from anhydrous solid sodium nitrite or anhydrous solid sodium nitrate weighed on an analytical balance to the nearest milligram and dissolved in ultra-pure de-ionized water, respectively.

        An example preparation of a nitrite solution would be: 2.0 ppm NO21- = 2.0 mg/L NO21- X 1.50 mg NaNO2/mg NO21- = 3.00 mg/L NaNO2 -- weigh 30.0 mg NaNO2 and dilute to volume in a 100 ml volumetric flask (0.300 mg/ml). Take 10 ml of this solution and dilute to volume in a 1 L volumetric flask (3.00 mg/L NaNO2).

        An example preparation of a nitrate solution would be: 20.0 ppm NO31- = 20.0 mg/L NO31- X 1.37 mg NaNO3/mg NO31- = 27.40 mg/L NaNO3 -- weigh 274.0 mg NaNO3 and dilute to volume in a 100 ml volumetric flask (2.74 mg/ml). Take 10 ml of this solution and dilute to volume in a 1 L volumetric flask (27.40 mg/L NaNO3).

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